Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

Domaines

Médecine vétérinaire et santé animale Virologie

Fichier principal

zjm287.pdf (467.27 Ko)

Origine	Fichiers éditeurs autorisés sur une archive ouverte
licence	Copyright (Tous droits réservés)

Stephan Zientara : Connectez-vous pour contacter le contributeur

https://normandie-univ.hal.science/hal-02946322

Soumis le : mardi 3 septembre 2024-21:43:56

Dernière modification le : lundi 16 septembre 2024-13:30:05

Dates et versions

hal-02946322 , version 1 (03-09-2024)

Licence

Identifiants

HAL Id : hal-02946322 , version 1
DOI : 10.1128/JCM.01935-14
PUBMED : 25339390
PUBMEDCENTRAL : PMC4290937

Citer

Aymeric Hans, Delphine Gaudaire, Jean-Claude Manuguerra, Albertine Léon, Antoine Gessain, et al.. Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus. Journal of Clinical Microbiology, 2014, 53 (1), pp.287-291. ⟨10.1128/JCM.01935-14⟩. ⟨hal-02946322⟩

Exporter

BibTeX XML-TEI Dublin Core DC Terms EndNote DataCite

Collections

PASTEUR ANSES CNRS INRA COMUE-NORMANDIE ENVA INRAE UP-SCIENCES VIRO EPI-PHYS-VIR-ONC PASTEUR_UMR3569

80 Consultations

2 Téléchargements